Scale-up of dextransucrase production by Leuconostoc mesenteroides in fed batch fermentation

Scale-up of dextransucrase production by Leuconostoc mesenteroides in fed batch fermentation

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Fed Bed Sheets batch fermentation was carried out for the dextransucrase enzyme production from Leuconostoc mesenteroides and the production was scale-up using oxygen transfer criteriuom.It was found that in 5 L vessel fermentation capacity, the best agitation speed was 225 min-1 and aeration rate was 0.15 vvm, obtaining dextransucrase activity of 127 DSU/mL.The maximum enzyme production velocity coincide with the maximum growth velocity between 6 and 7 h of fermentation, which confirmed that dextransucrase Soda production was associated with microbial growth.High enzyme yields were achieved during scale up based on oxygen transfer rate.